The localization of glycollate-pathway enzymes in Euglena
نویسندگان
چکیده
منابع مشابه
The localization of glycollate-pathway enzymes in Euglena.
Isolation of organelles from broken-cell suspensions of phototrophically grown Euglena gracilis Klebs was achieved by isopycnic centrifugation on sucrose gradients. 2. Equilibrium densities of 1.23g/cm3 for peroxisome-like particles, 1.22g/cm3 for mitochondria and 1.17g/cm3 for chloroplasts were recorded. 3. The enzymes glycollate dehydrogenase, glutamate-glyoxylate aminotransferase, serineglyo...
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1. Lowering of the concentration of carbon dioxide in air available to phototrophically growing Euglena cultures from 5% to the normal value (0.03%) resulted in an increased specific activity of glycollate oxidoreductase. 2. The effects of chloramphenicol and cycloheximide suggested that this increase in activity was due to enzyme synthesis de novo on cytoplasmic ribosomes. 3. The K(m) for glyc...
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متن کاملOccurrence and operation of the glycollate--glyoxylate shuttle in mitochondria of Euglena gracilis Z.
Both glyoxylate reductase (NADP+) and glycollate dehydrogenase were located exclusively in mitochondria in Euglena gracilis and constitute the glycollate--glyoxylate shuttle, whose existence in higher plants was thought doubtful, owing to different subcellular locations of the two enzymes. Disrupted Euglena mitochondria showed a glycollate-dependent NADPH oxidation, indicating actual operation ...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1975
ISSN: 0306-3283
DOI: 10.1042/bj1480321